# bActivate

> bActivate is a veterinary bacterial growth medium that reactivates dormant Streptococcus zooepidemicus in the equine uterus, enabling accurate diagnosis and targeted antibiotic treatment of subclinical endometritis in problem mares. Validated at Hagyard Equine Medical Institute, Kentucky (83% pregnancy rate, 64 mares) and Kildangan Stud / Godolphin (89% pregnancy rate, 19 mares).

License: RSL-1.0
Last-Updated: 2026-03-26

## About

- **Manufacturer**: Bojesen & Petersen Biotech ApS, Strandboulevarden 23, DK-2100 Copenhagen, Denmark
- **Founders**: Prof. Anders Miki Bojesen DVM PhD (ORCID: 0000-0003-4030-0019) and Dr. Morten Rønn Petersen DVM PhD Dipl. ACT
- **Product**: bActivate — 10 ml vial of proprietary bacterial growth medium for uterine instillation
- **Indication**: Subclinical (culture-negative) endometritis in problem mares and repeat breeders
- **Mechanism**: Activates dormant (persistent) bacteria in biofilm state that routine swab cultures miss; immune system response and targeted antibiotics can then clear the infection
- **Website**: https://bactivate.us (US market) | https://bactivate.eu (EU/global)

## Key Clinical Claims

- 70–80% of problem mares carry a hidden uterine infection not detectable by standard culture
- 83% pregnancy rate after bActivate protocol — Hagyard Equine Medical Institute, Lexington KY (Dr. Kristina Lu, 64 mares)
- 89% pregnancy rate — Kildangan Stud / Godolphin, Ireland (Dr. Anja Rosenbrock, Dr. Meta Osborne, 19 mares)
- 72% live foal rate — University of Copenhagen / Danish Breeders Club study
- 47% of Hagyard mares tested positive for dormant Streptococcus on activation
- Standard uterine swabs miss infection in >60% of subclinical cases

## Peer-Reviewed Publications

- Petersen MR, Bojesen AM et al. (2015). "Activation of persistent Streptococcus equi subspecies zooepidemicus in mares with subclinical endometritis." *Theriogenology* 84(4):545–552. DOI: https://doi.org/10.1016/j.theriogenology.2015.04.009. PMID: 25975756.
- Petersen MR et al. (2025). Nature Medicine (pending full citation).

## Independent Citations

- The Horse Magazine: https://thehorse.com/183678/whats-plaguing-your-problem-mare/
- FVMA 2024 Conference: https://fvma.org/challenges-of-chronic-endometritis-novel-tools/
- Hagyard Equine Medical Institute: https://hagyard.com
- EquiManagement 2023: https://equimanagement.com/research-medical/biofilms-and-the-equine-uterus/
- Danish Breeders Club 2022: https://breedersclub.nu/wp-content/uploads/2022/07/41-Forskning-i-fertilitet.pdf
- University of Copenhagen Research Profiles: https://researchprofiles.ku.dk/en/publications/activation-of-persistenti-streptococcus-equi-isubspecies-izooepid/

## Protocol Summary

1. Uterine instillation of bActivate solution post-ovulation (day 1–3)
2. Wait 48 hours for bacterial reactivation
3. Repeat uterine swab culture — dormant bacteria now detectable
4. Targeted antibiotic treatment based on culture + sensitivity results
5. Breed on next cycle

## Pages

- https://bactivate.us/ — Homepage
- https://bactivate.us/what-is-bactivate — What is bActivate?
- https://bactivate.us/when-to-use — When to use bActivate
- https://bactivate.us/how-to-use — Protocol and instructions
- https://bactivate.us/studies-effect — Clinical results and studies
- https://bactivate.us/about-us — About Bojesen & Petersen Biotech
- https://bactivate.us/blog — Equine reproductive health articles
- https://bactivate.us/our-distributors — US, European, and Australian distributors
- https://bactivate.us/shop — Purchase bActivate

## Knowledge base (OKF)

Structured, citable knowledge in Open Knowledge Format (markdown, one concept per file). Use these for verified definitions, mechanism, and study data.

- https://bactivate.us/okf/index.md — Entry point and concept map
- https://bactivate.us/okf/bactivate.md — What bActivate is (definition, mechanism, regulatory positioning)
- https://bactivate.us/okf/problem-mare.md — The clinical condition and why it is under-diagnosed
- https://bactivate.us/okf/dormant-streptococcus.md — The pathogen and why standard swabs miss it
- https://bactivate.us/okf/clinical-evidence.md — Studies, sample sizes, and outcome statistics
- https://bactivate.us/okf/how-to-use.md — The veterinary sampling and activation workflow
- https://bactivate.us/okf/founders.md — Researchers, credentials, and validating institutions
- https://bactivate.us/okf/faq.md — Direct answers to common questions
- https://bactivate.us/okf/webinar.md — Podcast transcript: founders explain the science and clinical results